Research Use Protocols
These are our recommended research protocols, provided to support proper preparation, handling, and administration in order to maintain peptide integrity and consistency in experimental use.
Tips & Best Practices (Step-by-Step)
Tips & Best Practices (Step-by-Step)
For educational/research use only. Not medical advice.
1) Prep & Hygiene
Wash hands and clean your workspace.
Gather: alcohol wipes (70% isopropyl), insulin syringes, diluent (e.g., bacteriostatic water if appropriate), peptide vial(s), and a sharps container.
Inspect the vial for cracks, discoloration, or particles.
2) Remove Cap & Disinfect
Flip off the vial’s plastic/metal safety cap (rubber stopper stays in place).
Scrub the stopper with an alcohol wipe for ~30 seconds and let it dry.
3) Reconstituting (Mixing Powder with Diluent)
Pull back the insulin syringe plunger to draw in the same amount of air as diluent you plan to add.
Insert the syringe into the vial and inject the air first (balances pressure).
Draw up the correct amount of diluent.
Slowly inject the diluent down the side wall of the vial—not directly onto the powder.
⚠️ Important: If you inject the liquid too forcefully, it can damage the peptide structure, leading to degradation or visible particles forming in the solution. Always add diluent gently.
Swirl the vial lightly until the solution is clear. Do not shake.
4) Understanding Syringe Units
Insulin syringes are usually U-100:
• 100 units = 1.00 mL
• 50 units = 0.50 mL
• 25 units = 0.25 mL
• 10 units = 0.10 mL
Formula:
Volume (mL) = Desired dose (mcg) ÷ Concentration (mcg/mL)
Units = Volume × 100
Example: 250 mcg from a 2,000 mcg/mL vial = 0.125 mL = 12.5 units.
5) Drawing Up a Dose
Wipe the stopper again with an alcohol wipe.
Pull back the syringe to draw air equal to the dose you’ll withdraw.
Insert, inject the air, invert the vial, and slowly draw up your dose.
Tap out bubbles and confirm the units.
Dispose of syringes safely in a sharps container.
6) Pressure Control
Always inject air before drawing liquid to avoid a vacuum.
If solution pushes out or syringe feels like it’s being sucked in, slow down and equalize pressure.
7) Storage & Handling
Store according to product instructions (many require refrigeration once mixed).
Avoid freeze–thaw cycles.
Discard if cloudy, discolored, or if persistent particles form after proper mixing.
8) Quick Troubleshooting
Particles/flakes: may mean peptide was damaged during reconstitution or has degraded — discard.
Fizzing/bubbles: usually from injecting diluent too quickly.
Rubber specks: caused by stopper coring — use a fine-gauge insulin syringe and insert smoothly.
AOD-9604 – Protocol
AOD-9604 – Protocol (Injection Solution)
What it is / Uses:
AOD-9604 is a 16–amino acid fragment of human growth hormone (hGH 176–191).
Investigated for its ability to stimulate fat metabolism (lipolysis and inhibiting lipogenesis) without raising IGF-1 like full growth hormone.
Studied for weight management, fat loss, and metabolic regulation.
Considered more stable for fat-loss research than full GH due to its targeted action.
Reconstitution:
Usually reconstituted with acetic acid (AA) solution rather than BAC water, as stability is better in AA.
Add diluent slowly down the glass wall, not directly onto the powder. Swirl gently until dissolved.
Concentration Example (for reference):
5 mg vial + 2 mL AA water → 2.5 mg/mL (2500 mcg/mL).
With U-100 insulin syringes, 0.1 mL = 250 mcg.
Typical Research Dosing Reported:
250–500 mcg once daily SubQ.
Often administered in the morning or pre-training, with cycles lasting 4–8 weeks.
Handling Notes:
AOD-9604 can become gel-like when cold. Let vial reach room temperature before drawing.
Do not shake; swirl gently. Injecting diluent too forcefully can damage the peptide and cause particles or cloudiness.
Store reconstituted solution in the fridge. Avoid repeated freeze–thaw cycles.
Bacteriostatic Water – Protocol
Bacteriostatic Water — Protocol (Injection Solution)
Reconstitution / Use:
Used as a diluent to reconstitute lyophilized (freeze-dried) peptides into injectable solution.
Contains 0.9% benzyl alcohol, which acts as a preservative and allows multi-use from the same vial.
Always inject BAC water slowly down the side of the peptide vial to avoid damaging fragile peptides. Swirl gently until dissolved.
Concentration Example (for reference):
If a 5 mg peptide vial is reconstituted with 2 mL BAC water → 2.5 mg/mL (2500 mcg/mL).
With U-100 insulin syringes: 0.1 mL = 250 mcg.
(Exact concentration depends on the volume of BAC water added.)
Typical Research Use Reported:
Commonly used for multi-dose reconstitution of peptides such as BPC-157, TB-500, Ipamorelin, CJC-1295 (no DAC), etc.
Not used as a “dose” itself — it is the carrier solution for peptides.
Handling Notes:
Refrigerate after first opening.
Safe to use for up to 28 days after first puncture (per manufacturer guidelines).
Always swab the stopper with alcohol before each withdrawal.
Not recommended for peptides that are unstable in BAC (e.g., AOD-9604, which prefers acetic acid).
Do not freeze. Discard if solution becomes cloudy or contaminated.
BPC-157 – Protocol
BPC-157 — Protocol (Injection Solution)
What it is / Uses:
Synthetic peptide fragment derived from gastric protein.
Studied for tendon and ligament repair, joint recovery, gut protection, reducing inflammation, and accelerated tissue healing.
Commonly combined in research with TB-500 for systemic recovery or with GHK-Cu for connective tissue, skin, and vascular support.
Reconstitution:
Typically reconstituted with Bacteriostatic Water (BAC).
Inject the diluent slowly down the side of the vial to avoid damaging the peptide.
Swirl gently until clear; do not shake.
Concentration Example (for reference):
5 mg vial + 2 mL BAC water → 2.5 mg/mL (2500 mcg/mL).
With U-100 insulin syringes: 0.1 mL = 250 mcg.
Typical Research Dosing Reported:
200–500 mcg once or twice daily SubQ.
Often administered near the site of injury in research settings.
Cycles reported in literature are usually 2–4 weeks, sometimes longer.
Handling Notes:
Store in the refrigerator after reconstitution.
Do not freeze; avoid repeated freeze–thaw cycles.
Discard if cloudy, discolored, or if flakes appear.
Use sterile technique at all times.
CJC-1295 (No DAC) – Protocol
CJC-1295 (No DAC) — Protocol (Injection Solution)
What it is / Uses:
CJC-1295 (no DAC), also called Mod GRF(1-29), is a synthetic peptide fragment that mimics growth hormone–releasing hormone (GHRH).
Unlike CJC-1295 with DAC (drug affinity complex), the no DAC version is short-acting, producing a natural GH pulse rather than prolonged elevation.
Studied for growth hormone stimulation, fat loss, muscle recovery, and sleep quality when combined with a GHRP (e.g., Ipamorelin).
Reconstitution:
Commonly reconstituted with Bacteriostatic Water (BAC).
Inject diluent slowly down the side of the vial to protect the peptide.
Swirl gently until dissolved; do not shake.
Concentration Example (for reference):
2 mg vial + 2 mL BAC water → 1 mg/mL (1000 mcg/mL).
With U-100 insulin syringes: 0.1 mL = 100 mcg.
Typical Research Dosing Reported:
100-250 mcg subcutaneously 2 times daily, often in the morning, pre-training, and/or before bed.
Frequently combined with Ipamorelin in research protocols for synergistic GH release.
Short cycles may last 4–12 weeks depending on the study design.
For greater GH spikes, research commonly advises avoiding carbohydrates for ~30 minutes before and after injections, since insulin can blunt GH release.
Handling Notes:
Store in the refrigerator after reconstitution.
Avoid freeze–thaw cycles.
It is preferred to not pre-mix in the same vial with other peptides (e.g., Ipamorelin) — this can lead to precipitation and reduced stability.
Discard if solution appears cloudy or develops flakes.
Delta Sleep-Inducing Peptide – Protocol
DSIP (Delta Sleep-Inducing Peptide) — Protocol (Injection Solution)
What it is / Uses:
DSIP is a naturally occurring neuropeptide that was first isolated from the brain in the 1970s.
It has been studied for its potential effects on sleep regulation, circadian rhythm, stress response, and hormone balance.
Research explores its role in improving sleep quality, reducing cortisol, and modulating growth hormone release.
Reconstitution:
Commonly reconstituted with Bacteriostatic Water (BAC).
Inject the diluent slowly down the vial wall to protect the peptide.
Swirl gently until fully dissolved. Do not shake.
Concentration Example (for reference):
5 mg vial + 2 mL BAC water → 2.5 mg/mL (2500 mcg/mL).
With U-100 insulin syringes: 0.1 mL = 250 mcg.
Typical Research Dosing Reported:
100–200 mcg subcutaneously shortly before sleep.
Some studies report nightly use over 2–4 weeks for sleep cycle regulation.
Often researched in conjunction with other peptides affecting GH or sleep pathways (e.g., GHRPs, CJC-1295).
Handling Notes:
Store in the refrigerator once reconstituted.
Avoid freeze–thaw cycles.
Discard if solution becomes cloudy or develops particles.
For best results in research settings, DSIP is usually administered prior to bedtime routines.
GHK-Cu – Protocol
GHK-Cu — Protocol (Injection Solution)
What it is / Uses:
GHK-Cu is a naturally occurring copper-binding peptide found in human plasma, saliva, and urine.
It is typically supplied as a light blue lyophilized powder due to its copper content.
Studied for its role in skin regeneration, wound healing, hair growth stimulation, collagen production, and anti-inflammatory activity.
Often researched for anti-aging and cosmetic applications, and sometimes paired with BPC-157 or TB-500 for connective tissue repair.
Reconstitution:
Commonly reconstituted with Bacteriostatic Water (BAC).
Slowly add diluent down the side of the vial to avoid peptide damage.
Swirl gently until dissolved; do not shake.
Concentration Example (for reference):
A standard vial contains 50 mg of GHK-Cu powder.
If reconstituted with 10 mL BAC water → 5 mg/mL (5000 mcg/mL).
With U-100 insulin syringes: 0.1 mL = 500 mcg.
Typical Research Dosing Reported:
Literature and research use often describes ranges from 2 mg up to 5 mg daily, depending on study design and application (systemic vs. localized).
Topical or cosmetic applications have used concentrations around 0.1%–2% in creams or gels.
Cycles may run 4–8 weeks for regenerative purposes.
Handling Notes:
The solution should maintain a blue tint after reconstitution — this is normal and reflects copper content.
Mild stinging is common due to the copper content. This can often be reduced by:
Diluting with a larger volume of BAC water, and/or
Allowing the vial to sit at room temperature for 10–15 minutes before use (injecting cold solution can increase sting).
Store in the refrigerator after reconstitution.
Do not freeze; avoid repeated freeze–thaw cycles.
Discard if the solution becomes cloudy, changes color significantly, or develops flakes.
HCG (Human Chorionic Gonadotropin) – Protocol
HCG (Human Chorionic Gonadotropin) — Protocol (Injection Solution)
What it is / Uses:
HCG is a naturally occurring glycoprotein hormone produced during pregnancy.
In research and clinical settings, it has been studied for male fertility, stimulating natural testosterone production, weight management, and hormonal support.
It is also used in some protocols alongside peptides or testosterone for gonadal axis regulation.
Reconstitution:
HCG usually comes in a 5000 IU lyophilized vial with a separate vial of diluent.
When reconstituting with Bacteriostatic Water (BAC), inject the diluent slowly down the side of the vial to preserve peptide integrity.
Swirl gently until fully dissolved; do not shake.
Concentration Example (for reference):
5000 IU vial + 5 mL BAC water → 1000 IU/mL.
With U-100 insulin syringes: 0.1 mL = 100 IU.
5000 IU vial + 2.5 mL BAC water → 2000 IU/mL.
With U-100 insulin syringes: 0.1 mL = 200 IU.
Typical Research Dosing Reported:
Studies report ranges from 250 IU – 2000 IU subcutaneously or intramuscularly, depending on design and application.
In fertility-related research, dosing is often repeated 2–3 times weekly.
When paired with TRT protocols, smaller frequent doses (e.g., 250–500 IU 2–3 times weekly) are used in research to maintain testicular volume and natural testosterone production.
With enclomiphene, HCG may be studied as a complementary agent to stimulate Leydig cell activity while enclomiphene supports FSH/LH release.
Research cycles may last several weeks to months depending on the purpose.
Handling Notes:
Store reconstituted HCG in the refrigerator.
Light-sensitive — keep vials protected from direct light.
Do not freeze; avoid repeated freeze–thaw cycles.
Discard if the solution becomes cloudy, develops flakes, or changes color.
Always use sterile syringes and swab vial tops before each use.
HGH (Somatropin) – Protocol
Human Growth Hormone (Somatropin) — Protocol (Injection Solution)
What it is / Uses:
Human Growth Hormone (HGH, Somatropin) is a synthetic form of the natural pituitary hormone.
Studied for its role in growth, cell repair, protein synthesis, fat metabolism, and recovery.
In research and clinical settings, HGH has been evaluated for GH deficiency, anti-aging, muscle preservation, and fat loss.
Reconstitution:
HGH typically comes lyophilized in multi-mg vials.
Reconstitute gently with Bacteriostatic Water (BAC) or sterile water (depending on manufacturer instructions).
Inject diluent slowly down the vial wall; swirl gently until clear.
Do not shake — HGH is extremely delicate and can denature.
Concentration Example (for reference):
10 IU vial reconstituted with 1 mL BAC water → 10 IU/mL (each 0.1 mL = 1 IU).
10 IU vial with 2 mL BAC water → 5 IU/mL (each 0.1 mL = 0.5 IU).
(Different vials may be labeled in mg or IU; 1 mg Somatropin = ~3 IU.)
Typical Research Dosing Reported:
Studies have used low daily doses (0.1–0.3 mg/day) in adults for GH deficiency, and higher ranges in HIV-wasting research (up to 0.1 mg/kg/day, max 6 mg/day).
In performance and anti-aging research, subcutaneous micro-doses are typically given once daily or split into two injections (AM and/or pre-bed).
For greater GH spikes, research often advises avoiding carbohydrates for ~30 minutes before and after injections, since insulin can blunt GH release.
Research cycles vary widely, ranging from several weeks to long-term studies.
Handling Notes:
Store reconstituted HGH in the refrigerator, protected from light.
Never freeze; avoid repeated freeze–thaw cycles.
Discard if solution becomes cloudy or develops particles.
Always use sterile syringes; swab vial tops before each withdrawal.
Ipamorelin – Protocol
Ipamorelin — Protocol (Injection Solution)
What it is / Uses:
Ipamorelin is a selective ghrelin receptor agonist (a GHRP, or Growth Hormone Releasing Peptide).
It is studied for its ability to stimulate GH release without significantly raising cortisol or prolactin, unlike older GHRPs.
Research explores its role in muscle recovery, fat metabolism, sleep quality, and synergistic GH release when paired with CJC-1295 (no DAC).
Reconstitution:
Commonly reconstituted with Bacteriostatic Water (BAC).
Inject diluent slowly down the side of the vial to protect peptide chains.
Swirl gently until dissolved; do not shake.
Concentration Example (for reference):
2 mg vial + 2 mL BAC water → 1 mg/mL (1000 mcg/mL).
With U-100 insulin syringes: 0.1 mL = 100 mcg.
Typical Research Dosing Reported:
Literature and research use often describe 100–200 mcg subcutaneously, 2–3 times daily.
Frequently combined with CJC-1295 (no DAC) for synergistic GH pulses.
In research settings, injections are often timed in the morning, pre-training, and/or before sleep.
For optimal GH release, studies and research protocols often recommend avoiding carbohydrates for ~30 minutes before and after injection, since insulin can blunt GH spikes.
Cycles may last 4–12 weeks depending on the study design.
Handling Notes:
Store in the refrigerator after reconstitution.
Do not freeze; avoid repeated freeze–thaw cycles.
Best advised not to pre-mix/store in the same vial with CJC-1295 — this can cause precipitation and reduced stability.
Discard if solution becomes cloudy, discolored, or develops particles.
Melanotan II – Protocol
Melanotan II — Protocol (Injection Solution)
What it is / Uses:
Melanotan II is a synthetic α-melanocyte–stimulating hormone (α-MSH) analog.
Studied for its ability to stimulate skin pigmentation (tanning), enhance photoprotection, and influence libido.
Research has also explored potential effects on appetite regulation and energy balance.
Reconstitution:
Typically reconstituted with Bacteriostatic Water (BAC).
Inject diluent slowly down the side of the vial to preserve peptide integrity.
Swirl gently until dissolved; do not shake.
Concentration Example (for reference):
10 mg vial + 2 mL BAC water → 5 mg/mL (5000 mcg/mL).
With U-100 insulin syringes: 0.1 mL = 500 mcg.
Typical Research Dosing Reported:
Research protocols often start with very low doses (50–250 mcg SubQ) to assess tolerance.
Doses may be gradually increased up to 250–500 mcg SubQ daily, depending on the research goal.
Once desired pigmentation is achieved, “maintenance” schedules in research may reduce frequency (e.g., 1–2 times per week).
Cycles may run for several weeks, often aligned with periods of sun exposure.
Handling Notes:
Store in the refrigerator after reconstitution.
Do not freeze; avoid repeated freeze–thaw cycles.
Commonly reported side effects in research include nausea, flushing, and increased libido, especially at higher doses.
Discard if solution becomes cloudy, develops flakes, or changes color.
Nasal Spray Kit – Protocol
Nasal Spray Kit — Protocol (Preparation & Use)
What it is / Uses:
A delivery system designed for intranasal administration of peptides such as Selank, Semax, or DSIP.
Provides a convenient alternative to subcutaneous injections, allowing peptides to be absorbed through the nasal mucosa.
Studied for applications in nootropic, anxiolytic, and sleep-related research.
Preparation:
Ensure all components (vial, diluent, and nasal spray bottle) are clean and sterile.
Reconstitute the peptide in its vial using Bacteriostatic Water (BAC). While BAC water may sting slightly, it is preferred because it helps keep the compound stable for longer.
Saline, boiled, or purified water may feel gentler, but peptides begin to degrade much faster when mixed with those, reducing shelf life.
Transfer the solution carefully into the nasal spray bottle using a sterile syringe.
If you add additional BAC water to dilute the spray, gently mix the bottle around so the solution distributes evenly — avoid shaking.
Prime the pump until a fine mist is released.
Concentration Example (for reference):
A typical nasal spray bottle holds 5–10 mL of solution.
If 5 mg of peptide is reconstituted into 5 mL, concentration = 1 mg/mL (1000 mcg/mL).
If the spray bottle delivers 0.1 mL per spray, then each spray = 100 mcg.
Typical Research Dosing Reported:
Research protocols vary depending on the compound (e.g., Selank/Semax often 200–400 mcg daily intranasally, split into multiple sprays).
Sprays are typically divided between nostrils for even absorption.
Research cycles may last from 1–4 weeks, depending on the study design.
Handling Notes:
Always store the filled spray bottle in the refrigerator unless otherwise directed by the peptide specifications.
Let the solution warm to room temperature before use to improve comfort and reduce nasal irritation.
Clean the nozzle regularly to avoid contamination or clogging.
Discard any solution that becomes cloudy, discolored, or older than the recommended use period.
Peptide Pen Kit – Protocol
Peptide Pen Kit — Protocol (Preparation & Use)
What it is / Uses:
A reusable injection device designed to deliver peptides accurately and with minimal waste.
Works with refillable cartridges (max 3 mL) that hold reconstituted peptide solution.
Allows for precise micro-dosing using U-100 insulin unit markings.
Commonly used in research with peptides like HGH, Ipamorelin, CJC-1295, BPC-157, and others.
Preparation:
Reconstitute your peptide in its vial using Bacteriostatic Water (BAC) (preferred for stability).
Place an empty cartridge inside the pen body before filling.
Draw up your peptide solution into a sterile insulin syringe.
Before injecting the solution, push a small amount of air from the syringe into the cartridge slowly. This helps balance pressure inside and prevents the grey seal from popping off.
After the air step, slowly inject the peptide solution into the cartridge while it is inside the pen. Injecting too quickly can still cause pressure spikes.
Do not exceed 3 mL per cartridge.
We do not recommend mixing multiple compounds into one cartridge — stability differs and one compound may degrade faster than the other.
Once filled, prime the pen until a droplet appears at the needle tip to confirm accuracy.
Always attach a new sterile pen needle before each injection.
Concentration Example (for reference):
If a 5 mg peptide is reconstituted with 2 mL BAC water → 2.5 mg/mL (2500 mcg/mL).
In a 3 mL cartridge, the pen delivers doses in insulin units (U-100 scale):
• 10 units = 0.1 mL = 250 mcg
• 20 units = 0.2 mL = 500 mcg
Typical Research Dosing Reported:
Varies depending on the peptide (e.g., HGH, Ipamorelin, CJC-1295).
The pen is not peptide-specific — it is a delivery tool for accurate and repeatable dosing.
Often used for daily micro-doses or split doses across the day.
Handling Notes:
Store filled cartridges in the refrigerator.
Let the pen or cartridge sit at room temperature 10–15 minutes before use to reduce injection sting.
Never reuse pen needles — always attach a fresh one.
Discard cartridges if the solution becomes cloudy, discolored, or past stability limits.
Wipe the outside of the pen with alcohol periodically; do not immerse in water.
Retatrutide – Protocol
Retatrutide — Protocol (Injection Solution)
What it is / Uses:
Retatrutide is a triple agonist peptide (GIP, GLP-1, and glucagon receptor) being studied for weight management, type 2 diabetes, and metabolic health.
It helps by reducing appetite, stabilising blood sugar, and increasing energy expenditure.
Early research shows it may produce greater weight loss than semaglutide or tirzepatide in comparable timeframes.
Reconstitution:
Supplied as a lyophilised powder.
Reconstitute with Bacteriostatic Water (BAC).
Add the diluent slowly down the vial wall and swirl gently until dissolved — do not shake.
Concentration Example (for reference):
10 mg vial + 2 mL BAC water → 5 mg/mL.
With a U-100 insulin syringe:
• 10 units (0.1 mL) = 0.5 mg
• 20 units (0.2 mL) = 1 mg
Typical Research Dosing Reported:
Suggested titration from literature and reported use:
• Start at 1 mg subQ once weekly
• Gradually increase to 2 mg once weekly after several weeks
• Most research subjects report the “sweet spot” between 2–3 mg weekly
Longer-term studies have escalated higher, but for most purposes 2–3 mg once weekly is considered effective.
Handling Notes:
Inject subcutaneously (subQ) into abdomen, thigh, or upper arm.
Rotate injection sites weekly to avoid irritation.
Store reconstituted solution in the fridge (2–8 °C). Avoid freezing or repeated freeze–thaw cycles.
If the solution turns cloudy or particles form, discard.
Selank – Protocol
Selank — Protocol (Intranasal)
What it is / Uses:
Selank is a synthetic peptide analog of tuftsin, studied for its anti-anxiety, nootropic, and neuroprotective effects.
Research indicates potential benefits for stress reduction, mood enhancement, cognitive support, and immune modulation.
Unlike benzodiazepines, it does not appear to cause sedation or dependence in research settings.
Reconstitution:
Supplied as lyophilised powder in 10 mg vials.
Reconstitute with 5 mL Bacteriostatic Water (BAC).
Add diluent slowly down the vial wall and swirl gently until dissolved — do not shake.
Concentration Example (for reference):
10 mg vial + 5 mL BAC water → 2 mg/mL (2000 mcg/mL).
Typical Research Dosing Reported (Intranasal):
200–400 mcg per spray session.
1–2 times daily, only when needed.
Research cycles usually last 2–4 weeks, depending on study design.
Handling Notes:
Tilt head back slightly when spraying, alternating nostrils if using multiple sprays.
Store reconstituted vial in the fridge (2–8 °C).
Avoid freezing and repeated freeze–thaw cycles.
If adding extra BAC water to the spray device, gently rotate or tip to mix — do not shake vigorously.
Discard if solution becomes cloudy, discoloured, or forms particles.
Semax – Protocol
Semax — Protocol (Intranasal)
What it is / Uses:
Semax is a synthetic peptide derived from adrenocorticotropic hormone (ACTH 4–10 fragment), studied for its neuroprotective, nootropic, and neuroregenerative properties.
Research suggests benefits for cognitive enhancement, focus, memory support, recovery after brain injury, and neuroinflammation reduction.
It is often studied in similar contexts as Selank but with a stronger emphasis on cognition and neuroprotection.
Reconstitution:
Supplied as a lyophilised white powder in 10 mg vials.
Reconstitute with 5 mL Bacteriostatic Water (BAC).
Add diluent slowly down the vial wall and swirl gently until dissolved — do not shake.
Concentration Example (for reference):
10 mg vial + 5 mL BAC water → 2 mg/mL (2000 mcg/mL).
Typical Research Dosing Reported (Intranasal):
200–400 mcg per spray session.
1–2 times daily, only when needed.
Research protocols often run for 2–4 weeks, with some extending longer depending on the study.
Handling Notes:
Tilt head back slightly when spraying, alternating nostrils if using multiple sprays.
Store reconstituted vial in the fridge (2–8 °C).
Avoid freezing and repeated freeze–thaw cycles.
If adding extra BAC water to the spray device, gently rotate or tip to mix — do not shake vigorously.
Discard if solution becomes cloudy, discoloured, or forms particles.
TB-500 – Protocol
TB-500 — Protocol (Injection Solution)
What it is / Uses:
TB-500 (Thymosin Beta-4 fragment) is a synthetic peptide fragment studied for its tissue repair, wound healing, angiogenesis, and recovery support.
Research suggests benefits for muscle and tendon repair, joint recovery, anti-inflammatory effects, and accelerated healing from injury.
It is often stacked with BPC-157 for enhanced healing and recovery outcomes.
Reconstitution:
Supplied as a lyophilised white powder (typically in 5 mg vials).
Reconstitute with Bacteriostatic Water (BAC).
Add diluent slowly down the vial wall and swirl gently until dissolved — do not shake.
Concentration Example (for reference):
5 mg vial + 2 mL BAC water → 2.5 mg/mL (2500 mcg/mL).
Typical Research Dosing Reported:
Loading phase: 2–2.5 mg injected twice weekly for the first 4–6 weeks.
Maintenance phase: 2–2.5 mg injected once every 1–2 weeks.
Administration is typically subcutaneous (SC) or intramuscular (IM) depending on study design.
Handling Notes:
Inject slowly and gently to avoid peptide degradation or particle formation.
Store reconstituted solution in the fridge (2–8 °C).
Do not freeze; avoid repeated freeze–thaw cycles.
Discard if solution becomes cloudy, discoloured, or develops particles.
Tesamorelin – Protocol
Tesamorelin — Protocol (Injection Solution)
What it is / Uses:
Tesamorelin is a synthetic growth hormone–releasing hormone (GHRH) analog.
Clinically approved for reducing visceral adipose tissue in HIV-associated lipodystrophy.
In broader research it is studied for GH stimulation, fat metabolism, body composition improvements, and metabolic health.
Reconstitution:
Typically supplied as a lyophilized powder in single-use vials (commonly 2-5 mg).
Reconstituted with the provided diluent or Bacteriostatic Water (BAC).
Inject diluent slowly down the side of the vial to protect the peptide.
Swirl gently until clear; do not shake.
Concentration Example (for reference):
2 mg vial + 1 mL BAC water → 2 mg/mL (2000 mcg/mL).
With U-100 insulin syringes: 0.1 mL = 200 mcg.
Typical Research Dosing Reported:
Clinical literature uses 2 mg subcutaneously once daily.
Often administered in the evening before bed, aligning with natural GH release.
Research commonly notes that avoiding carbohydrates for ~30 minutes before and after injection may help optimize GH spikes, since insulin can blunt GH release.
Study durations range from several weeks to many months, depending on design.
Handling Notes:
Store reconstituted Tesamorelin in the refrigerator, protected from light.
Do not freeze; avoid repeated freeze–thaw cycles.
Discard if the solution becomes cloudy, develops flakes, or changes color.
Use sterile syringes and swab vial tops before each withdrawal.
